human tnf alpha elisa research reagent kit picokine  (Boster Bio)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Tianma Granules Alleviate AOM / DSS ‐Induced Colorectal Tumorigenesis by Inhibiting the Wnt/β‐Catenin Pathway Activation

doi: 10.1111/jcmm.70772

Figure Lengend Snippet: Tianma granules alleviated the pathological damage and inflammation of CRC mice and promoted CRC cell apoptosis. (A) H&E staining was used to detect the pathological damage of colon tissues (black arrows indicated tumours in the colon). (B) AB‐PAS staining was used to detect and quantify goblet cell numbers in colon tissues. (C) TUNEL staining was used to detect cell apoptosis and the apoptotic cell rate in colon tumour tissues (green fluorescence represented apoptotic cells). (D–G) ELISA was used to detect the levels of inflammatory cytokines (TNF‐α, IL‐1β, IL‐6 and IFN‐γ) in colon tissues. (H) Western blot was used to detect the protein levels of CRC‐specific proteins (COX2 and MUC2) and apoptotic proteins (Bax, Cleaved‐caspase3 and Bcl2). N = 3. Data are expressed as mean ± SD. Statistical significance was determined by one‐way ANOVA followed by Tukey's multiple comparisons test. ** p < 0.01, compared with the control group; # p < 0.05, ## p < 0.01, compared with the CRC group.

Article Snippet: TNF‐α (CSB‐E04744m), IL‐1β (CSB‐E08054m) and IL‐6 (CSB‐E04639m) ELISA kits were purchased from Wuhan CUSABIO Biotech Co. Ltd. (Wuhan, Hubei, China); INF‐γ (PI507) ELISA kit was supplied by Shanghai Beyotime Biotechnology Co. Ltd. (Shanghai, China).

Techniques: Staining, TUNEL Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: ACS Bio & Med Chem Au

Article Title: 5′-Phosphorothioester Linked Cyclic Dinucleotides, Endo-S-CDNs, Displaying Impressive Antitumor Activities In Vivo when Dosed Subcutaneously

doi: 10.1021/acsbiomedchemau.5c00070

Figure Lengend Snippet: Figure 3. Comparison of induction of some proinflammatory cytokines in either THP1 cells or PMBC. (A) mRNA expression levels of CXCL10 in THP1 cells. (B) mRNA expression levels of TNFα in THP1 cells. (C) mRNA expression levels of IFNβ in THP1 cells. (D) Expression of TNFα in PMBCs after treatment with endo-S-CDNs as quantified using an ELISA assay. The mRNA expression levels were determined by real-time quantitative PCR (RT-qPCR). Statistical significance was performed using Students t-test (paired, one-tailed) with significant differences as * P ≤ 0.05.

Article Snippet: CDNs were treated at 40 μM, after 24 h cells were applied on human TNF alpha ELISA research reagent kit Picokine (from Boster Bio) per manufacturer’s instruction.

Techniques: Comparison, Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, One-tailed Test